Applied Entomology and Zoology (2017) 52, 567-579

Parveen G. Ansari, Rakesh K. Singh, Shruti Kaushik, Ashok Krishna, Takashi Wada and Hiroaki Noda (2017)
Detection of symbionts and virus in the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae), vector of the Mungbean yellow mosaic India virus in Central India
Applied Entomology and Zoology 52 (4), 567-579
Abstract: Legume crops in Central India, the main soybean production area of the country, may suffer from yellow mosaic disease caused by the Mungbean yellow mosaic India virus (MYMIV). MYMIV is transmitted by the sweet potato whitefly, Bemisia tabaci (Gennadius), which is a species complex composed of various genetic groups. This vector species harbors different endosymbionts among regional strains and among individuals. To elucidate fundamental aspects of this virus vector in the state of Madhya Pradesh, the infection status of the symbionts and the virus in whiteflies was studied. A polymerase chain reaction (PCR) survey of the whiteflies collected in Madhya Pradesh found four secondary endosymbionts, Arsenophonus, Hemipteriphilus, Wolbachia, and Cardinium, in addition to the primary endosymbiont Portiera. Arsenophonus and Hemipteriphilus were highly infected but the infection rates of Wolbachia and Cardinium were low. MYMIV was detected in whitefly populations collected from various host plants in Madhya Pradesh. The whitefly populations belonged to the Asia I and II genetic groups; several different Asia II populations were also distributed. Specific relations were not observed among symbiont infection status, virus infection, and the whitefly genetic groups in the populations of Madhya Pradesh, though Cardinium was highly detected in the Asia II-1 group. New primers, which can be used for PCR template validation and for discriminating two phylogenetically close endosymbionts, were designed.
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Link to article at publishers website
Database assignments for author(s): Takashi Wada

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Mungbean yellow mosaic India virus		India
	Bemisia tabaci biotype Asia II 1		India
	Bemisia tabaci biotype Asia I		India