Applied Entomology and Zoology (2015) 50, 317-329

Dararat Hotaka, Alongkorn Amnuaykanjanasin, Chan Maketon, Siraprapa Siritutsoontorn and Monchan Maketon (2015)
Efficacy of Purpureocillium lilacinum CKPL-053 in controlling Thrips palmi (Thysanoptera: Thripidae) in orchid farms in Thailand
Applied Entomology and Zoology 50 (3), 317-329
Abstract: Ten entomopathogenic fungi were tested for the control of female adult Thrips palmi Karny in the laboratory. The most virulent fungus Purpureocillium lilacinum CKPL-053, two commercial bioinsecticides and a chemical insecticide were tested for control of T. palmi in two Dendrobium sp. orchid farms. Controls of T. palmi in both farms were highest with P. lilacinum CKPL-053. Purpureocillium lilacinum CKPL-053 and the Odashima strain P. lilacinum produced paecilotoxins A, B, and C when cultured in complete medium 1 but not in potato dextrose broth. Jasmonic acid contents in Dendrobium sp. petals after application of P. lilacinum CKPL-053, B.bassiana CKB-048, M. robertsii CKM-048, and P. lilacinum CKPL-012 spore suspensions were significantly higher than with imidacloprid and water control treatments. This suggests that jasmonic acid might assist in activating plant defense mechanism to exhibit the repellence and/or resistance effect of Dendrobium sp. against T. palmi. The LC₅₀ and LT₅₀ for P. lilacinum CKPL-053 against female T. palmi were 2.67 × 10⁵ conidia ml^-1 and 2.6 days at the concentration of 1 × 10⁸ conidia ml^-1, respectively.
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Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Thrips palmi	Dendrobium (genus)	Thailand
Purpureocillium lilacinum (entomopathogen)	Thrips palmi	Dendrobium (genus)	Thailand