Applied Entomology and Zoology (1999) 34, 9-17

J.Y. Wang, T. Iwasaki and Y. Aizono (1999)
A 40-kilodalton protein with growth inhibitory activity against the adzuki bean weevil in seeds of Vigna mungo
Applied Entomology and Zoology 34 (1), 9-17
Abstract: A proteinous factor inhibiting the growth of the adzuki bean weevil (Callosobruchus chinensis L.) was separated from the seeds of Vigna mungo L. Hepper (urd bean), by sequential fractionations with ammonium sulfate, chromatographies on DEAE-cellulose, hydroxyapatite and Q-Sepharose columns. The proteinous factor was further purified to homogeneity by chromatography with a Mono-P column. The purified factor was a 40-kDa single polypeptide, as determined by SDS-PAGE, and heat-labile but stable over a pH range from 4 to 6. It is noteworthy that this factor showed neither alpha-amylase and protease inhibitor activities nor lectin activity, in contrast to other growth inhibitors with those activities against bean weevils. The growth inhibitory activity was lost by respective digestions with alpha-chymotrypsin and subtilisin, indicating that the 40-kDa protein was a novel growth inhibitor in urd beans against the adzuki bean weevil.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Callosobruchus chinensis		Japan