Applied Entomology and Zoology (1998) 33, 349-355

O. Khamiss, X. Lery, M.H. Belal, H.A. Badawy, J. Gianotti and S.M. Abol-Ela (1998)
Effects of some insecticides on the division of a Spodoptera littoralis cell line and on the replication of Sl Baculovirus (NPV)
Applied Entomology and Zoology 33 (3), 349-355
Abstract: The impact of several insecticides has been studied on a Spodoptera littoralis cell line and their effects on the replication of S. littoralis nucleopolyhedrovirus (Sl NPV). Four chemical insecticides, Chloropyrifos, Fenitrothion, Cypermethrin and Carbaryl which belong to three different groups of pesticide, organophosphorus, pyrethroid, and carbamate, respectively, were used. The results demonstrate an increase of more than 25 % in the multiplication of Sl cells when treated with Chloropyrifos dilutions compared with untreated cells. The most destructive pesticide was Cypermethrin, which caused 84% inhibition of cell development (0.4 x 10⁶ cell/ml) at a concentration of 10^-3µg/ml in preliminary tests. In the presence of both Sl NPV and sublethal doses of these insecticides, the TCID₅₀ values revealed that the Cypermethrin (10^-8µg/ml) was the most toxic (ID₅₀ was 6.48 x 10⁵/ml) whereas Chloropyrifos was the least toxic (ID₅₀ 9:29 x 10¹²/ml) at a concentration of 0.731 µg/ml. The PFU values confirm the results that the same concentration of Chloropyrifos (0.731 µg/ml) was the least toxic (PFU 6.41 x 10¹³/ml) and Cypermethrin was the most toxic (4.47 x 10⁶/ml PFU) for the other concentrations as well as the different insecticides the PFU values ranged between 2.8 x 10⁸ for Chloropyrifos at a concentration of 10^-3 µg/ml and 9.76 x 10¹² for Carbaryl at 10^-5µg/ml. A comparison with the results obtained using Sl NPV without chemicals (the PFU 3.5 x 10⁹/ml) indicates an increase in the production of virus and titer, and a synergistic effect of the chemicals. These results confirm the effect of these insecticides in vivo. As the Cypermethrin may have a potent effect on the exchange of ions through the cell membrane in vitro and in vivo, it could partially explain the impact of the insecticides and their mode of action.
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Database assignments for author(s): Said M. Abol-Ela

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
control - general
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera littoralis
Alphabaculovirus spliturae (entomopathogen)