Apidologie (2003) 34, 195-206

Elke Genersch and Christoph Otten (2003)
The use of repetitive element PCR fingerprinting (rep-PCR) for genetic subtyping of German field isolates of Paenibacillus larvae subsp. larvae
Apidologie 34 (3), 195-206
Abstract: Studies using the repetitive element PCR fingerprinting technique (rep-PCR) revealed that BOX A1R-, MBO REP1- and ERIC-primers reproducibly generate distinctive DNA fingerprints from Paenibacillus larvae subsp. larvae. Four different genetic subtypes of P. larvae were identified in Germany using the three primers. Correlating the results from genetic and morphological subtyping, the flat and transparent morphotype could be assigned to one genetic group. Geographic evaluation of our results demonstrated that the different genetic subtypes appeared in clusters correlating with different outbreaks of American foulbrood. Taken together, our results indicated that rep-PCR performed with a combination of BOX A1R- and MBO REP1-primers will be an effective tool for establishing a molecular epidemiology of P. l. larvae.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Elke Genersch

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Paenibacillus larvae		Germany