Anzeiger für Schädlingskunde, Pflanzenschutz, Umweltschutz (1995) 68, 175-178

S. Messerschmid and S. Sarkar (1995)
Nachweis des Nelkenadernscheckungsvirus (CVMV) mit verschiedenen ELISA-Systemen
[Identification of Carnation vein mottle virus (CVMV) with different ELISA-systems]
Anzeiger für Schädlingskunde, Pflanzenschutz, Umweltschutz 68 (8), 175-178
Abstract: The direct double antibody sandwich-ELISA (DAS) and the indirect protein A sandwich-ELISA (PAS-ELISA) were compared for the detection of carnation vein mottle virus (CVMV). The PAS-ELISA turned out to be much more sensitive than the direct system. The virus could be detected significantly in a 1:10.000- or 1:50.000-dilution of plant sap of CVMV-infected pinks (Dianthus barbatus), whereas with DAS the virus could be measured significantly only in a 1:100-dilution. Using four different enzymes as markers (alkaline phosphatase, horseradish peroxidase, penicillinase, ß-D-galactosidase) linked with protein A as conjugate in PAS, there was no difference concerning the detection limit. However, DAS was found to be much more specific than PAS-ELISA.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: German)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Carnation vein mottle virus	Dianthus (genus)