Annals of the Entomological Society of America (2022) 115, 105-112

Raymond Yokomi, Jennifer K. Delgado, Thomas R. Unruh, Nina M. Barcenas, Stephen F. Garczynski, Spencer Walse, Adalberto A. Pérez de León and William Rodney Cooper (2022)
Molecular advances in larval fruit moth identification to facilitate fruit export from western United States under systems approaches
Annals of the Entomological Society of America 115 (1), 105-112
Abstract: Molecular advances facilitate fruit export by improving rapid pest diagnosis by polymerase chain reaction (PCR) and advanced sequencing technology. Improved pest detection provides timely certification of the quarantine pest-free status in the commodity being exported, avoiding unnecessary commodity treatment. The U.S.–Japan Systems Approach to export fresh cherries from the Western United States that targets the codling moth, Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae), is used as an example. Suspect codling moth larvae interdicted at cherry packing houses are distinguished by PCR from other internal fruit moth larvae such as the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae); lesser appleworm, G. prunivora (Walsh) (Lepidoptera: Tortricidae); cherry fruitworm, G. packardi (Zeller) (Lepidoptera: Tortricidae); and filbertworm, Cydia latiferreana (Walsingham) (Lepidoptera: Tortricidae). Identification is confirmed by sequencing the amplicon of a 301 bp region of the COI gene produced by PCR of the DNA from a suspect moth and comparing this sequence of COI gene sequences of other internal fruit feeders of pome fruit. This sequence comparison results in unambiguous pest identification. These findings are discussed in the context of systems approach research to meet evolving needs of phytosanitary requirements for global export of fruits.
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Database assignments for author(s): Raymond K. Yokomi, Thomas R. Unruh, W. Rodney Cooper

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cydia pomonella	Cherry (Prunus avium - Prunus cerasus)	U.S.A. (SW)
	Cydia pomonella	Cherry (Prunus avium - Prunus cerasus)	U.S.A. (NW)