Annals of the Entomological Society of America (2000) 93, 291-294

F. Zeng, S. Shu, S.B. Ramaswamy and A. Srinivasan (2000)
Vitellogenin in pupal hemolymph of Diatraea grandiosella (Lepidoptera: Pyralidae)
Annals of the Entomological Society of America 93 (2), 291-294
Abstract: Vitellogenin (Vg) concentration was determined in pupal hemolymph of female southwestern corn borer, Diatraea grandiosella (Dyar). Vitellogenin concentration increased significantly with pupal age. Vitellogenin level reached 7 mg/ml on day 7 after pupation. The vitellogenin was separable into 2 apoproteins on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with molecular weights of 145,000 for apoVg-I and 38,000 for apoVg-II. SDS-PAGE analysis revealed that vitellogenin polypetides in female pupal hemolymph appeared to be identical to those from eggs, but were absent in male and early female pupal hemolymph. A method to quantify vitellogenin by indirect ELISA is described. Results from this study and those of a previous study suggest that juvenile hormone does not have a role in vitellogenesis but does so in choriogenesis.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Sonny B. Ramaswamy

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Diatraea grandiosella