Annals of Applied Biology (2005) 147, 89-100

A. Akad, E. Teverovsky, D.Y. GidoniElad, B. Kirshner, D. Rav-David, H. Czosnek and G. Loebenstein (2005)
Resistance to Tobacco mosaic virus and Botrytis cinerea in tobacco transformed with complementary DNA encoding an inhibitor of viral replication-like protein
Annals of Applied Biology 147 (1), 89-100
Abstract: We have previously cloned a 1016-bp-long complementary DNA (cDNA) clone (named NC330) encoding an inhibitor of virus replication (IVR)-like protein from induced-resistant leaves of Nicotiana tabacum cv. Samsun NN. In the present work, NC330 cDNA was cloned in an Agrobacterium binary vector and introduced into the genome of N. tabacum cv. Samsun nn that is susceptible to Tobacco mosaic virus (TMV). Eight R₀ were highly resistant, with TMV titres less than 2% of the controls. The progeny of these primary transformants resulted in four TMV-resistant and four susceptible lines. The progeny of the plant with highest resistance for TMV was selfed during four generations. In the R₂ generation, out of 112 plants tested, 3 plants showed excellent resistance and 5 a lesser degree of resistance. NC330 transcripts were detected in these R₂ plants as well as in their R₁ and R₀ parents. Two R₂ plants were self-pollinated to obtain the R₃ generation. All the 25 R₃ progeny plants of one line contained PCR-amplifiable NC330, compared with only 6 of the 23 progeny plants from the other line. Following TMV inoculation, virus was undetectable in seven of the eight tested R₃ progeny of the first line and five of the six R₃ progeny plants of the second line. NC330 transcripts were detected in the TMV-resistant transgenic plants but also in some susceptible plants. TMV resistance was suppressed when the plants were kept at higher temperatures (32-34°C), whereas returning the plants to lower temperatures (24-26°C) restored resistance. Transgenic plants resistant to TMV were also resistant to B. cinerea following inoculation of seedlings or of whole plants. The presence of the NC330 DNA was correlated with changes in the physiology of the transgenic plant. Seed germination was inhibited at high temperatures and in the dark. Rootlets of germinating seeds of the transgenic plants grew significantly faster than those of control seeds. These observations were correlated with a significantly higher concentration of abscisic acid in the seeds and seedlings of the transgenic plants. In silico analysis suggests that the NC330 protein translated from the NC330 cDNA is a tetratricopeptide repeats protein and that it may be involved in protein-protein interaction as part of the pathogen resistance mechanism.
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Link to article at publishers website
Database assignments for author(s): Gad Loebenstein, Henryk Czosnek

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea	Tobacco (Nicotiana tabacum)
	Tobacco mosaic virus	Tobacco (Nicotiana tabacum)