Anais da Sociedade Entomológica do Brasil (1997) 26, 375-378

José E. Serrão, Marta da Silva, Carlos R. Sousa-Silva and Josué M. Pacheco (1997)
Uso de Serologia na Identificação de Predadores de Orphulella punctata (De Geer) (Orthoptera: Acrididae)
[Use of serology to identify predators of Orphulella punctata (De Geer) (Orthoptera: Acrididae)]
Anais da Sociedade Entomológica do Brasil 26 (2), 375-378
Abstract: Serological techniques were used to study Orphulella punctata (De Geer) predators. For preparation of antiserum, rabbits were immunized by the limphonodule injection method with antigens obtained from O. punctata male. Serological tests were performed using double diffusion in agar gel. Specific serological reactions were obtained 13 days after the 1st inoculation. After the 2nd inoculation 5 precipitation lines were observed. In the reactions with female antigens there were 2-4 lines, while egg antigens showed 2 lines. These variations in the number of lines were attributed to different physiological states and to ontogeny. Positive reactions were observed for spiders and chrysopids captured in the field.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Database assignments for author(s): José Eduardo Serrao

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Orphulella punctata