Agronomía Tropical (2006) 56, 555-559

Carolina Bernal, Ivan Galindo, Delis Perez and Nardy Diez (2006)
Aplicación de la proteómica comparativa para la identificación de proteínas en Phaseolus vulgaris asociadas a resistencias a plagas
[Application of comparative proteins in Phaseolus vulgaris associated with pest resistance]
Agronomía Tropical 56 (4), 555-559
Abstract: The attack of coleopteran from the bruchidae family represent one of the most important risks that farmers confront, lowering crop yield and bean quality of Phaseolus vulgaris, and affecting the production costs of this important food staple in many traditional diets. Several authors describe the presence of storage proteins from the lectins family (Phytohemagglutinins, PHA; Arcelin, Arc; and α-amylase inhibitor, αAI) as insecticidal proteins, representing a very effective means to control pest attacks during storage on many varieties of P. vulgaris. However, these investigations focus their analysis on one protein at a time, without taking into account their functional interrelationships. The present work is aided by proteomics to analyze insecticidal protein expressions in various cultivated P. vulgaris varieties. The use of 2D-immunedetection of specific insecticidal proteins has shown to be a simplified procedure to initially establish functional correlations between this group of proteins and the observed bruchid resistance phenotype.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Spanish)

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Acanthoscelides obtectus	Stored legumes
	Callosobruchus maculatus	Stored legumes