Agricultural and Forest Entomology (2003) 5, 219-225

Andrew G.S. Cuthbertson, Colin C. Fleming and Archie K. Murchie (2003)
Detection of Rhopalosiphum insertum (apple-grass aphid) predation by the predatory mite Anystis baccarum using molecular gut analysis
Agricultural and Forest Entomology 5 (3), 219-225
Abstract: 1 A simple, yet sensitive polymerase chain reaction based technique was developed for the detection of the apple-grass aphid Rhopalosiphum insertum in the gut of Anystis baccarum, a predatory mite.
2 A range of conserved polymerase chain reaction primers for insect mitochondrial and ribosomal DNA were tested in order to amplify R. insertum DNA. The mitochondrial DNA primers LrRNAR2 + N1F1, amplified a region between the ND1 and large subunit RNA genes.
3 DNA sequencing of the R. insertum ND1-LRNA polymerase chain reaction product allowed aphid-specific polymerase chain reaction primers to be designed. These amplified a 283-bp product from individual aphids. No polymerase chain reaction product was amplified from individual A. baccarum.
4 Using the aphid-specific primers against A. baccarum fed on R. insertum, the diagnostic 283-bp product was amplified.
5 Two restriction enzymes (RsaI and AluI) produced patterns that allowed unambiguous identification of R. insertum DNA from that of Macrosiphum euphorbiae and Myzus persicae.
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Full text of article
Database assignments for author(s): Andrew G.S. Cuthbertson, Archie K. Murchie

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Rhopalosiphum insertum		United Kingdom
Anystis baccarum (predator)	Rhopalosiphum insertum		United Kingdom