Acta Phytopathologica et Entomologica Hungarica (2019) 54, 173-186

M.W. Abbasi, M.Q. Khan, M.J. Zaki, S.S. Shaukat, A. Rauf, N. Ahmed, M. Azeem and M. Tariq (2019)
Soil application of Bacillus thuringiensis Berliner isolates against root-knot nematode (Meloidogyne javanica (Treub) Chitwood) in okra (Abelmoschus esculentus (L.) Moench)
Acta Phytopathologica et Entomologica Hungarica 54 (2), 173-186
Abstract: Bacillus thuringiensis (B.t) is well known for its biocontrol potential against a variety of insects. Nematicidal potential of ten B.t isolates was tested against root-knot nematodes (Meloidogyne javanica (Treub) Chitwood) in vitro, under greenhouse as well as in field conditions. Eggs and second stage juveniles (J2) were exposed to 5 and 25% concentrations of bacterial cell-free aqueous extracts up to 96 h. B.t isolates showed lesser degrees of nematicidal activity at 5% concentration. However, some B.t isolates (B.t-14, B.t-16 and B.t-64) greatly reduced egg hatching and increased J2. All B.t isolates revealed suppressed egg hatching and increased mortality of J2 at 25% concentration. Soil applications with most of the B.t isolates under greenhouse and field conditions significantly improved height and fresh weights of root-knot nematode parasitized okra (Abelmoschus esculentus (L.) Moench). Some isolates, including B.t-64 reduced the number of galls and egg masses. B.t-64 reduced gall formation up to 70% under greenhouse conditions. However, 29% of decrease was observed in field conditions. Similarly, B.t-64 treated plants showed a 56% decreased in eggs/egg mass in a field experiment. Population of root-knot nematodes in the rhizosphere was decreased up to 61% in the field experiment as compared to control.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Syed Shahid Shaukat

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne javanica	Okra and relatives (Abelmoschus)
Bacillus thuringiensis (antagonist)	Meloidogyne javanica	Okra and relatives (Abelmoschus)