Acta Entomologica Sinica (2008) 51, 1304-1308

Qi Qiao, Hai-Chao Li, Guo-Hui Yuan, Xian-Ru Guo and Mei-Hao Luo (2008)
[Preparation of the antiserum to HassG-alpha-q of Helicoverpa assulta (Lepidoptera : Noctuidae) and its immunolocalization in the antenna]
Acta Entomologica Sinica 51 (12), 1304-1308
Abstract: The objective of this study is to localize G protein alpha-q subunit of Helicoverpa assulta (HassGaq) in the male antennae for the further research about the role of HassGaq in olfactory signal transduction in H. assulta. Balb/c mice were immunized with HassGaq protein expressed in prokaryotic cells to produce antiserum. The prepared antiserum and the purchased anti-Gq/11alpha antiserum were used in Western blot after male antennal homogenate of Helicoverpa assulta was separated by SDS-PAGE. The results indicated that the prepared antiserum could react with HassGaq, and also the non-target proteins, suggesting that several transcripts of HassGaq or other G protein a subunits may be expressed in male antennae. In addition, anti-Gq/11alpha antiserum could detect HassGaq with high specificity. Accordingly, the expression sites of HassGaq in adult male antennae were examined by immunocytochemical method using anti-Gq/1 la antiserum. The results revealed that HassGaq was associated with trichodic sensilla and basiconic sensilla as immunolabelling were typically observed inside and at the base of the two kinds of sensilla. It is so inferred that HassGaq may be involved in olfactory signal transduction in H. assulta.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Guo-Hui Yuan

Research topic(s) for pests/diseases/weeds:
pheromones/attractants/traps

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa assulta