Acta Entomologica Sinica (2007) 50, 762-768

Gao-Hui Qiu and Zhao-Jun Han (2007)
[Cloning and sequence analysis of nicotinic acetylcholine receptor subunits from the grain aphid, Sitobion avenae (Fabricius)]
Acta Entomologica Sinica 50 (8), 762-768
Abstract: Nicotinic acetylcholine receptor (nAChR) is one of the most important insecticide targets. In this study, five alpha-subunit genes from the grain aphid, Sitobion avenae (Fabricius) were cloned to their full-length with RT-PCR and RACE strategy. It was also found that the alpha-5 subunit had two forms with only difference in a 175 bp fragment. Sequence analysis showed that these genes had all the characteristics of nAChR gene family and were high homology with the reported nAChR genes from other insects. This work facilitates the further studies on natural subunit composition of nAChR and the molecular mechanisms for target resistance to new nicotinic insecticides in this pest.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zhaojun Han

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sitobion avenae