Acta Entomologica Sinica (2007) 50, 474-480

Qian Luo, Xia Feng, Li-Hua Lu, Shu-Qing Wan and Huan-Yu Chen (2007)
[Linkage mapping of resistance to abamectin in the diamondback moth using AFLP marker]
Acta Entomologica Sinica 50 (5), 474-480
Abstract: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important insect pest in cruciferous vegetables and has developed high resistance to avermectin all over the world. In this study, AFLP was used to construct a linkage map of resistance to avermectin in diamondback moth. The mapping families consisted of the susceptible strain, the abamectin-resistant strain and their backcross progeny. A total of 1 044 DNA bands and 271 polymorphic loci were amplified using 10 combinations of AFLP primers. Chi-square (Chi²) analysis indicated that only 123 bands fit the segregation ratio of 1:1 (P=0.05) in these polymorphic loci. Twenty-eight linkage groups were constructed with 112 bands, covering about 1 222.7 cM of the genomics of the diamondback moth. Three markers of E1M4-15, E1M1-4, and E1M4-2, linked with abamectin-resistant gene were situated on the same linkage group and their distance apart from resistant gene was 0 cM, 8.3 cM, and 13.1 cM, respectively. Comparison with the constructed linkage map showed that the gene was located on the fifth linkage group. These AFLP markers associated with resistance to abamectin show a strong potential of application in monitoring diamondback moth resistance to abamectin.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Lihua Lu

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella