Acta Entomologica Sinica (2006) 49, 768-774
Guang-Xi Guo, Yong Liu, Jing-Juan Yang and Xiang-Zhen Ma (2006)
[Identification, activity and function determination of several salivary enzymes secreted by Macrosiphum avenae]
Acta Entomologica Sinica 49 (5), 768-774
Abstract: Parafilm method was adopted for feeding the third instar nymphs of Macrosiphum avenae on two liquid diets (20% sucrose and redistilled water). Their saliva after feeding for 24 h was collected and assayed identifying salivary enzymes and determining their activities. The results showed that pectinase, polyphenol oxidase and cellulase were identified in 20% sucrose diet; cellulase was identified in redistilled water diet; peroxidase was not identified in both diets. The activities of pectinase, polyphenol oxidase and cellulase secreted into 20% sucrose diet by 30 aphids were 2.59×10-3 U/g, 7×10-3 U/g and 7.89×10-3 U/g, respectively; and cellulase activity secreted into redistilled water diet by 30 aphids was 3.68×10-3 U/g. In the behavioral response test, the predator Coccinella septempunctata and parasitoid Aphidius avenae of M. avenae showed much preference for the volatiles in wheat seedlings treated by pectinase. It was so concluded that pectinase secreted by M. avenae play an important role in wheat plant indirect defense induced by aphid feeding.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Yong Liu
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
environment/habitat manipulation
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Sitobion avenae | Wheat (Triticum) | |||
| Coccinella septempunctata (predator) | Sitobion avenae | Wheat (Triticum) | ||
| Aphidius picipes (parasitoid) | Sitobion avenae | Wheat (Triticum) |
