Acta Entomologica Sinica (2005) 48, 833-836

Zhi-Gang Liu, Meng Li, Ying Bao, Ren-Long Fu and Dong-Ming Su (2005)
[Antigenic localization of Der p1 in the body of the house dust mite, Dermatophagoides pteronyssinus by immunohistochemistry]
Acta Entomologica Sinica 48 (6), 833-836
Abstract: The localization of Der p1 in the body of the house dust mite, Dermatophagoides pteronyssinus was studied. Der p1 antibody as primary antibody was prepared by immunizing the mice with Der p1 antigen. The fluorescent antimouse IgG antibody was used as secondary antibody. D. pteronyssinus was embedded in paraffin, sectioned, and then reacted with the primary and secondary antibody. The location of Der p1 in the body was observed with fluoroscope. Some sections were stained with HE staining. A large part of body cavity of D. pteronyssinus was occupied by the digestive system as shown with HE staining. The Der p1 allergen was mostly localized in the midgut and gut contents, while the cuticle and reproductive system showed negative reaction in the immunostaining.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Dermatophagoides pteronyssinus