Acta Entomologica Sinica (2005) 48, 455-459

Lai Chen, Zu-Jian Wu, Guo-Sheng Fu, Qi-Ying Lin and Lian-Hui Xie (2005)
[Development of an in vitro culture system of primary tissues and cells from embryo of Laodelphax striatellus Fallen]
Acta Entomologica Sinica 48 (3), 455-459
Abstract: To build cell layer line of Laodelphax striatellus Fallen, seven different full culture media, including MM medium, lactalbumin hydrolysate, yeast extract and fetal bovine serum (FBS) based on Grace medium, were developed and tested to culture the tissues and cells from embryo of L. striatellus. The results indicated that these tissues and cells could survive keeping close to the bottom of medium for at least 1-4 weeks, and for even 2 months in the medium No. 5, which was made up of one Grace medium, one MM medium respectively and 20% FBS. Furthermore, effective hydrolyzation of the tissues was reached when they were pretreated for 5-10 min with 0.25% trypsin under the condition of pH 8.0 at 37°C. The suitable culture recipe to the tissues and cells from embryo of L. striatellus and a good method to separate tissues were proposed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zu-Jian Wu

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Laodelphax striatellus