Acta Entomologica Sinica (2003) 46, 553-557

Pei Liang, Feng-Xia Meng, Xi-Wu Gao, Bing-Zong Zheng and Gang Cheng (2003)
Biochemical characteristics of tyrosinase in the diamondback moth, Plutella xylostella (L.)
Acta Entomologica Sinica 46 (5), 553-557
Abstract: The optimum reaction conditions, sensitivity to the inhibitor and the developmental changes of tyrosinase in the diamondback moth, Plutella xylostella (L. ), were studied using time-driving dynamic method. The results showed that the optimum pH of tyrosinase is about 6.4. The OD₄₂₀ values are linear with time from 0 - 2 min. When stored at 22°C and 4°C, the prepared tyrosinase could keep its best activity for 12 h and 72 h, respectively; and while stored at - 20°C , the tyrosinase could keep its activity for at least 7 days. The activity of tyrosinase in different developmental stages of P. xylostella follows the order pharate pupa > 4th instar larvae > pupa > 1st -3rd instar larvae > adult. The l₅₀ of phenyl thiourea (PTU) to the activity of tyrosinase from both the resistant and susceptible strains showed no obvious difference (1.1796 µmol/L and 1.2795 µmol/L, respectively).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Xi-Wu Gao, Pei Liang

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella