Acta Entomologica Sinica (2003) 46, 549-552

Chun-Xi Ge, Jiong-Lie Huang, Guan-Jin Chen, Yu Wu and Hong-Feng Yu (2003)
Construction of transgenic vector pB [PUBnls-EGFP-prM] and identification of its integrational function in mosquito cell
Acta Entomologica Sinica 46 (5), 549-552
Abstract: To explore a new method to control arthropod-borne diseases, prM gene from dengue virus was inserted into a transposon piggyBac based plasmid and an insect transgenic vector pB [PUBnls-EGFP-prM] was constructed. The vector was used to transfect Aedes albopictus C6/36 cell with a helper plasmid. PCR and Southern blot tests showed that EGFP-prM gene had integrated into C6/36 cell genome. The results indicate that transposon piggyBac element is functional in Ae. albopictus and should be useful in further constructing dengue-refractory transgenic Ae. albopictus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aedes albopictus