Acta Entomologica Sinica (2002) 45, 724-727

Lu-Juan Sun, Xi-Wu Gao and Bing-Zong Zheng (2002)
Characteristics of carboxylesterase in omethoate-resistant and susceptible strains of cotton aphid, Aphis gossypii
Acta Entomologica Sinica 45 (6), 724-727
Abstract: A strain of organophosphate (OP)-resistant cotton aphid, Aphis gossypii was selected by exposure to omethoate insecticide in the laboratory. The resistance factor of this strain was 17 times that of an OP-susceptible strain of cotton aphid. Four substrates, alpha-naphthyl acetate (alpha-NA), alpha-naphthyl butyrate (alpha-NB), alpha-naphthyl phosphate (alpha-NP) and ß-naphthyl phosphate (ß-NP), were used to determine carboxylesterase activity, Michaelis constants (K_m) and maximum velocities (V_max) in OP-susceptible and resistant strains of cotton aphids. Carboxylesterase activity was significantly lower in the OP-resistant strain than the OP-susceptible strain when alpha-NA and ß-NB were used as substrates and the same trends were obtained by native polyacrylamide gel electrophoresis (PAGE) analysis. However, no significant difference was found between OP-susceptible and resistant strains when alpha-NP and ß-NP were used as substrates.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Xi-Wu Gao

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis gossypii