Acta Entomologica Sinica (1999) 42, 257-262

Jinfu Wang, Rui Chen, Shaohong Lu and Jiannong Xu (1999)
[PCR identification and probability of amplified esterase genes in Culex pipiens pallens]
Acta Entomologica Sinica 42 (3), 257-262
Abstract: Organophosphate insecticide (OP) resistance in mosquitoes of Culex pipiens complex has been shown to be correlated with the increase in esterase activity resulting from overproduction of the esterase due to amplification of the corresponding structural gene: Several esterase B loci have been identified with Southern hybridization and restriction fragment length polymorphism (RFLP). In this paper, esterase B loci in four OP-resistant strains of Culex pipiens pallens were identified with the restriction analysis of PCR fragments. The results indicate that various esterase loci have different selective advantages under different OP pressures. B₁ gene was amplified in temephos-resistant strain, and B₂ gene was amplified in trichlorphon and chlorpyrifos-resistant strains. The hybridzygote of B₁ and B₂ genes was detected in the malathion-resistant strain. Different natural populations of C. pipiens pallens have different probability of B gene distribution. These results are discussed in relation to the selective advantage of esterase genes to OP pressure and mosquito control strategies.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Jiannong Xu

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex pallens