Acta Entomologica Sinica (1998) 41, 225-230

Qiaohua Kang, Nianchun Chen, Shunwu Zhou and Shunzhang Qi (1998)
[Amplification of housefly P-450 cDNA by PCR directed by a pair of low G/C% content primers]
Acta Entomologica Sinica 41 (3), 225-230
Abstract: Based on multiplicity and genetic polymorphism of P-450 genes, total mRNA was isolated through oligo (dT) -cellulose column from total RNAs of an insecticide-susceptible strain of the housefly, Musca domestica vicina Macquart treated with sodium phenobarbital in the drinking water. Double-stranded cDNA were synthesized by reverse-transcription and used as template for polymerase chain reaction (PCR). The 1. 5kb long anticipated DNA fragment coding for P-450 protein was amplified by PCR, using a pair of low G/C% content primers designed according to CYP6A1 cDNA gene.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Musca domestica