Acta Entomologica Sinica (1997) 40, 128-134

Jing Wang, Jiagui Yuan, Yunqin Sun and Fawag Abadalla El-Said (1997)
[Technique for identification of insensitive acetylcholinesterase in resistant individuals of diamondback moth]
Acta Entomologica Sinica 40 (2), 128-134
Abstract: Acetylcholinesterase insensitivity is an important mechanism of resistance of diamondback moth (Plutella xylostella (L. )) to organophosphorus and carbamate insecticides, which has been widely recognized and documented. A dot-blot on nitrocellulose membranes technique for identification of insensitive AChE in single insects provides the opportunity for early detection and subsequent monitoring of resistance in field population. The technique is simple, sensitive and inexpensive. The percentages of inhibition of AChE activities in resistant strain (GBR) and field population (DG) were 50.97% and 43.96% respectively in the presence of propoxur and were 63.76% and 35.87% respectively in the presence of paraoxon. The results showed the GBR and DG had a higher degree of ACNE insensitivity to OP and carbamate than did the susceptible strain.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella