Pest Management Science (2016) 72, 1794-1802

Oderlei Bernardi, Daniel Bernardi, Renato J. Horikoshi, Daniela M. Okuma, Leonardo L. Miraldo, Julio Fatoretto, Fernanda C.L. Medeiros, Tony Burd and Celso Omoto (2016)
Selection and characterization of resistance to the Vip3Aa20 protein from Bacillus thuringiensis in Spodoptera frugiperda
Pest Management Science 72 (9), 1794-1802
Abstract:
BACKGROUND
Spodoptera frugiperda is one the main target pests of maize events expressing Vip3Aa20 protein from Bacillus thuringiensis (Bt) in Brazil. In this study, we selected a resistant strain of S. frugiperda on Bt maize expressing Vip3Aa20 protein and characterized the inheritance and fitness costs of the resistance.
RESULTS
The resistance ratio of the Vip3Aa20-resistant strain of S. frugiperda was >3200-fold. Neonates of the Vip3Aa20-resistant strain were able to survive and emerge as fertile adults on Vip3Aa20 maize, while larvae from susceptible and heterozygous strains did not survive. The inheritance of Vip3Aa20 resistance was autosomal recessive and monogenic. Life history studies to investigate fitness cost revealed an 11% reduction in the survival rate until adult stage and a ~50% lower reproductive rate of the Vip3Aa20-resistant strain compared with susceptible and heterozygous strains.
CONCLUSION
This is the first characterization of S. frugiperda resistance to Vip3Aa protein. Our results provide useful information for resistance management programs designed to prevent or delay resistance evolution to Vip3Aa proteins in S. frugiperda.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Oderlei Bernardi, Celso Omoto

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance to pesticides

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera frugiperda	Maize/corn (Zea mays)
Bacillus thuringiensis Vip-toxins (entomopathogen)	Spodoptera frugiperda	Maize/corn (Zea mays)