Nematology (2021) 23, 577-585

Ziga Laznik, Ivana Majic, Stanislav Trdan, Antoinette P. Malan, Annika Pieterse and Jenna L. Ross (2021)
Is Phasmarhabditis papillosa (Nematoda: Rhabditidae) a possible biological control agent against the Spanish slug, Arion vulgaris (Gastropoda: Arionidae)?
Nematology 23 (5), 577-585
Abstract: In the period from August to October 2018, 140 specimens of the Spanish slug, Arion vulgaris, were collected from Podbrezje, Slovenia. Slugs were dissected and examined for the presence of parasitic nematodes within the cadavers. Identification of the nematodes was conducted using morphological and molecular techniques and confirmed the presence of Phasmarhabditis papillosa. This is the first record of P. papillosa from the mollusc host, A. vulgaris. Laboratory experiments aimed at testing the efficacy of P. papillosa against A. vulgaris were conducted using nematodes grown in vivo. Nematodes were applied at concentration rates of 50, 100 and 200 nematodes slug^-1, respectively. Three weeks following treatment, the mortality of slugs was confirmed in all treatments (50 nematodes slug^-1, 37.4 ± 2.7%; 100 nematodes slug^-1, 48.4 ± 2.7%; 200 nematodes slug^-1, 50.6 ± 2.7%). However, the pathogenesis of P. papillosa was observed first in the treatments with the lowest nematode dose at 4 days after treatments, while a decrease in the feeding behaviour of slugs was noted first in the treatments with the highest nematode dose. Future opportunities for the potential use of P. papillosa as a biological control agent against slugs are discussed. This is the first report of P. papillosa from Slovenia, and of its virulence against A. vulgaris.
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Link to article at publishers website
Database assignments for author(s): Ziga Laznik, Stanislav Trdan, Antoinette P. Malan

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Arion vulgaris		Slovenia
Phasmarhabditis papillosa (pathogen)	Arion vulgaris		Slovenia