Mycologia (2007) 99, 693-704

Nathalie Gauthier, Cécile Dalleau-Clouet, Jacques Fargues and Marie-Claude Bon (2007)
Microsatellite variability in the entomopathogenic fungus Paecilomyces fumosoroseus: genetic diversity and population structure
Mycologia 99 (5), 693-704
Abstract: The hyphomycete Paecilomyces fumosoroseus (Pfr) is a geographically widespread fungus capable of infecting various insect hosts. The fungus has been used for the biological control of several important insect pests of agriculture. However knowledge of the fungus' genetic diversity and population structure is required for its sustainable use as a biological control agent. We investigated length and sequence polymorphisms of nine microsatellite loci for 33 Pfr accessions sampled from various host species and geographical locations, and our results reveal complex mutational processes for these molecular markers. Only Pfr isolates from Bemisia tabaci were amplified successfully, indicating the existence of Pfr genotypes specifically associated with B. tabaci. Genetic relationships among the 25 Pfr isolates from B. tabaci were inferred from allelic variability data at eight microsatellite loci that were polymorphic and subsequently from sequence data from the flanking regions of three selected loci. Maximum parsimony and neighbor joining analyses partitioned Pfr genetic diversity in two major lineages. One lineage included genotypes from the B-biotype of B. tabaci distributed across the Americas and was strongly supported in both analyses. Another lineage was distributed across Asia and consisted of four distinct clusters. Allele size homoplasy was found at the three microsatellite loci. We obtained better discrimination and resolution of the relationships among isolates with sequence data, although not all isolates could be typed. Thus sequencing of microsatellite flanking regions and repeats is a promising approach for the identification of Pfr isolates that specifically infect certain B. tabaci biotypes and phylogeographic studies.
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Link to article at publishers website
Database assignments for author(s): Nathalie Gauthier, Jaques Fargues, Marie-Claude Bon

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bemisia tabaci biotype MEAM1
Cordyceps fumosorosea (entomopathogen)	Bemisia tabaci biotype MEAM1