Australian Journal of Entomology (2007) 46, 305-312

Isabel Valenzuela, Ary A. Hoffmann, Mallik B. Malipatil, Peter M. Ridland and Andrew R. Weeks (2007)
Identification of aphid species (Hemiptera: Aphididae: Aphidinae) using a rapid polymerase chain reaction restriction fragment length polymorphism method based on the cytochrome oxidase subunit I gene
Australian Journal of Entomology 46 (4), 305-312
Abstract: The identification of immature aphids is often difficult or impossible. This can be a problem when there is a need for a rapid and accurate diagnosis of any aphid life stage, such as for quarantine inspections and horticultural surveys. A polymerase chain reaction restriction fragment length polymorphism technique is described on the mitochondrial cytochrome oxidase subunit I (COI) gene to develop a molecular identification key for immature aphids from Victoria, Australia. The restriction enzymes HpyCH4 IV, DraI, HinfI, TaqI and SspI characterised 26 haplotypes that corresponded to 25 aphid species commonly found in southern Australian aphid surveys, including the currant-lettuce aphid Nasonovia ribis-nigri (Mosley) that has recently invaded Australia, presumably from New Zealand. Overseas specimens of Aulacorthum solani (Kaltenbach) and N. ribis-nigri showed no significant sequence difference when compared with their Australian counterparts. The COI gene provides a useful marker for diagnostic aphid surveys.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Isabel Valenzuela, Ary A. Hoffmann

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aulacorthum solani
	Nasonovia ribisnigri		Australia (South+SE)