Australasian Plant Pathology (2015) 44, 619-627

F.A. Tanweer, M.Y. Rafii, K. Sijam, H.A. Rahim, F. Ahmed, S. Ashkani and M.A. Latif (2015)
Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families
Australasian Plant Pathology 44 (6), 619-627
Abstract: The study was conducted to identify the suitable SSR markers in relation to blast disease of rice. Backcross breeding was applied and BC₂F₁ generation was produced using rice variety Pongsu Seribu 2 as blast resistant parent and MR219 as susceptible parent. Eleven SSR markers related to blast resistance genes (Pi gene) were found highly polymorphic between the parental lines. According to single-gene model only two markers, RM208 and RM206, fitted to the expected test cross ratio (1:1) in BC₂F₁ generation with the relation to resistant and susceptible plants. Further BC₂F₁ population carrying 320 plants were inoculated with the highly virulent pathotype P7.2 of Magnaporthe oryze. Chi-square (chi2) test for single-gene model showed goodness of fit (p = 0.45) to the expected segregation ratio (1:1) for phenotypic segregation in BC₂F₁ population. In marker segregation analysis two markers RM208 (chi2 = 1.513, p = 0.218) and RM206 (chi2 = 0.613, P = 0.433) clearly produced goodness of fit to the expected test cross ratio (1:1) for the single-gene model. The present finding could help the rice breeders to monitor the blast resistance genes with appropriate and highly segregating SSR markers in rice varietal development program against blast disease.
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Link to article at publishers website
Database assignments for author(s): Mohd. Y. Rafii, Sadegh Ashkani, Mohammad Abdul Latif

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pyricularia oryzae	Rice (Oryza)	Malaysia