Phytopathology (1997) 87, 1168-1173

Alice K. Inoue-Nagata, Richard Kormelink, Tatsuya Nagata, Elliot W. Kitajima, Rob Goldbach and Dick Peters (1997)
Effects of temperature and host on the generation of Tomato spotted wilt virus defective interfering RNAs
Phytopathology 87 (11), 1168-1173
Abstract: The generation of defective interfering (DI) RNA molecules of tomato spotted wilt tospovirus (TSWV) was studied by serially passaging inocula from plant to plant under different controlled conditions. DI RNAs were generated at higher rates in plants at 16°C than in plants incubated at higher temperatures. Another factor promoting the TSWV DI RNA generation was the use of high virus concentrations in the inocula. The solanaceous species Capsicum annuum, Datura stramonium, Lycopersicon esculentum, Nicotiana benthamiana, and N. rustica supported the generation of DI RNAs, whereas the virus recovered from the inoculated composite species, Emilia sonchifolia, remained free of any DI RNA under all conditions tested. This study resulted in a strategy to maintain DI RNA-free TSWV isolates, as well as in an efficient way to produce a large population of different DI RNA species. A single DI RNA species usually became dominant in an isolate after a few rounds of serial inoculations. The possible mechanisms involved in TSWV DI RNA generation under different inoculation circumstances are discussed.
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Database assignments for author(s): Dick Peters, Tatsuya Nagata, Elliot W. Kitajima, Alice Kazuko Inoue-Nagata

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Orthotospovirus tomatomaculae