Pest Management Science (2021) 77, 4741-4748

Emre Inak, Esengül Özdemir, Abdullah Emre Atis, Filiz Randa Zelyüt, Arda Inak, Ünver Demir, Emmanouil Roditakis and John Vontas (2021)
Population structure and insecticide resistance status of Tuta absoluta populations from Turkey
Pest Management Science 77 (10), 4741-4748
Abstract:
BACKGROUND
Tuta absoluta is a devastating pest in tomato production areas worldwide. After its first introduction to Turkey in 2009, it quickly became the major pest of tomato-growing areas. Although some biocontrol agents have been used, especially in greenhouses, the main control of T. absoluta relies heavily on chemical insecticides. However, failure in chemical control has often been reported due to resistance development. In this study, we investigated (i) the population structure of 22 T. absoluta populations across Turkey by analysing haplotypes, based on the cytochrome oxidase subunit I gene; (ii) the efficacy of three registered insecticides from different classes (metaflumizone, chlorantraniliprole and spinosad) in real field-greenhouse conditions; and (iii) the geographic distribution of target-site mutations associated with insecticide resistance.
RESULTS
The efficacy of spinosad was higher than that of chlorantraniliprole and metaflumizone in the greenhouse trials, as documented by the mortality rates obtained, up to 14 days post application. Known resistance mutations in ryanodine receptors (RyR) (i.e. the I4790M/K and G4946E), nicotinic acetylcholine receptors (G275E), acetylcholinesterases (A201S) and voltage-gated sodium channels (F1845Y and V1848I) were found at various frequencies across the populations genotyped. The I4790K diamide resistance mutation in the RyR has been reported for the first time in T. absoluta populations. Although a total of eight haplotypes were found, the overall mean genetic distance was lower than 0.001, indicating the high genetic homogeneity among Turkish T. absoluta populations.
CONCLUSION
The results will contribute to design area-wide resistance management programs in T. absoluta control in Turkey. However, more monitoring studies are needed to implement evidence-based insecticide resistance management strategies in the frame of integrated pest management.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Emmanouil Roditakis, John Vontas

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phthorimaea absoluta	Tomato (Solanum lycopersicum)	Turkey