Applied Entomology and Zoology (2002) 37, 117-125

Arlene G. Bertuso and Sumio Tojo (2002)
The nature and titer of juvenile hormone in the brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae) in relation to wing morphogenesis and oocyte development
Applied Entomology and Zoology 37 (1), 117-125
Abstract: Whole body extracts from nymphs and adults of the brown planthopper, Nilaparvata lugens were analyzed for the known insect juvenile hormones (JHs) by gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. JH was separated successively by solvent extraction, chromatography on an aluminum oxide column and high performance liquid chromatography. JH III was identified as the only type of JH in both stages of this species. As the levels were very low for quantitative determination of JH III by GC-MS, we prepared haemolymph juvenile, hormone binding protein (JHBP) of Locusta migratoria which had a high affinity for JH III and used it in a competitive binding assay to quantify JH III in the haemolymph of the brachypterous and macropterous pure lines of N. lugens. However, the JH III titer developmental profiles were similar in the brachypterous and macropterous fifth (final) stadium nymphs. JH III titers in the haemolymph of the brachypters were significantly higher than those of macropters, coinciding with earlier development of oocytes in the brachypters, which supported the regulation of ovarian development by JH III.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Sumio Tojo

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Nilaparvata lugens		Japan