Forests (2021) 12 (3 - 362)

Colin L. Myrholm, Bradley D. Tomm, Renate Heinzelmann, Nicolas Feau, Richard C. Hamelin, Rebecca McDougal, Richard C. Winkworth and Tod D. Ramsfield (2021)
Development of a rapid loop-mediated isothermal amplification assay for the detection of Dothistroma septosporum
Forests 12 (3 - 362)
Abstract: A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.
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Database assignments for author(s): Richard C. Hamelin

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Dothistroma septosporum	Pine (Pinus)	U.S.A. (SW)
	Dothistroma septosporum	Pine (Pinus)	New Zealand
	Dothistroma septosporum	Pine (Pinus)	U.S.A. (NW)
	Dothistroma septosporum	Pine (Pinus)	U.S.A. (Alaska)
	Dothistroma septosporum	Pine (Pinus)	Canada (west)