Florida Entomologist (2013) 96, 850-858

Rafael Braga Da Silva, Ivan Cruz, Maria De Lourdes Corrêa Figueiredo, Wagner De Souza Tavares, José Eduardo Serrão and José Cola Zanuncio (2013)
Development and reproduction of Olla v-nigrum (Coleoptera: Coccinellidae) fed Anagasta kuehniella (Lepidoptera: Pyralidae) eggs supplemented with an artificial diet
Florida Entomologist 96 (3), 850-858
Abstract: Olla v-nigrum Mulsant (Coleoptera: Coccinellidae) preys on eggs and larvae of Coleoptera and Lepidoptera and on aphids in arboreal plants. Alternative prey supplemented with artificial diets may be used to mass rearing Coccinellidae predators for biological control programs. This study assessed the development and reproduction of O. v-nigrum fed Anagasta kuehniella Zeller (Lepidoptera: Pyralidae) eggs supplemented with an artificial diet. Adults of O. vnigrum were collected from Caesalpinia peltophoroides Benth. (Fabales: Fabaceae) and taken to the laboratory. Three male + female couples were fed on A. kuehniella eggs and an artificial diet (100 g of yeast, 40 g of honey, 0.5 g of ascorbic acid and 60 mL of water). Eight male + female couples obtained from this initial generation were subjected to the same procedure in order to assess the effects on subsequent generations. The mean number of eggs per egg mass was 11.7 ± 0.7 with a viability of 54.8 ± 2.9% and an incubation period of 3.1 ± 0.02 days. The durations of instars I, II, III and IV and the durations of the larval, pre-pupal, pupa and larva to adult stages were 2.4 ± 0.3, 1.8 ± 0.2, 2.0 ± 0.3, 5.8 ± 0.1, 12.1 ± 0.3, 1.0 ±0.01, 4.0 ±0.2 and 17.1 ± 0.5 days, respectively. The viabilities of the larval, pre-pupal and pupal stages of this predator were 46.3 ± 4.5%, 90.0 ± 5.0% and 100%, respectively. The diet and other methods employed in this study were adequate for laboratory rearing of O. v-nigrum but, because of the low viabilities of the eggs and immature stages observed with these methods, further improvements will be necessary for successful mass rearing of this valuable predator.
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Full text of article
Database assignments for author(s): José Cola Zanuncio, José Eduardo Serrao, Wagner de S. Tavares, Ivan Cruz

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Olla v-nigrum (predator)			Brazil (south)