BioControl (2020) 65, 297-304

James R. Hagler, Miles T. Casey and Scott A. Machtley (2020)
A procedure for pinpointing cannibalism, intraguild predation, and life stage-specific feeding events
BioControl 65 (3), 297-304
Abstract: A predator gut analysis technique is described that can simultaneously pinpoint predation events which are life stage-specific, intraspecies-specific (cannibalism) and interspecies-specific (intraguild). The third and fifth larval life stages of green lacewing, Chrysoperla carnea Stephens s.l. (Neuroptera: Chrysopidae), were marked with rabbit IgG and chicken IgY, respectively. The uniquely marked lacewing life stages were then introduced into caged arenas (n = 59 caged experimental units) containing a cotton plant and an assemblage of generalist predators. The predators released into each arena were recaptured after 6 h and their gut contents were examined for the presence of rabbit IgG- and chicken IgY-marked lacewing remnants by an anti-rabbit and anti-chicken enzyme-linked immunosorbent assay (ELISA), respectively. The predator gut ELISAs detected one cannibalism event and 14 and 8 intraguild predation (IGP) events on third and fifth instar lacewings, respectively. This proof-of-concept study shows that this universal prey immunomarking technique (UFIT), when combined with field cage methods, can be useful for pinpointing cannibalism and life stage-specific predation events.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): James R. Hagler

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Chrysoperla carnea (predator)