Journal of General Virology (1997) 78, 2095-2099

C. Vaquero, Y.C. Liao, J. Nahring and R. Fischer (1997)
Mapping of the RNA-binding domain of the Cucumber mosaic virus movement protein
Journal of General Virology 78 (8), 2095-2099
Abstract: A series of in-frame deletion mutants was used to identify a domain within the 3a protein of cucumber mosaic virus (CMV) that is required for RNA-binding activity. Deletions in the 3a gene were generated by PCR and restriction digestion, and the resulting mutated 3a sequences were cloned either in pT7-7 or in pGEX-5X3 expression vectors. The mutated 3a proteins or fusions with glutathione S-transferase (GST) were expressed in E. coli, purified, and their nucleic acid-binding activities analysed by photochemical UV cross-linking assays using digoxigenin-UTP-labelled RNA probes. Comparative analyses of seven mutated 3a proteins obtained from inclusion bodies and eight GST fusion proteins revealed that there is an RNA-binding domain located between amino acids 174 and 233. This RNA-binding domain is able to bind single- stranded RNA out of the context of the complete 3a movement protein and is highly conserved within both subgroups of CMV.
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Database assignments for author(s): Yu-Cai Liao

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus