Plant Disease (2016) 100, 976-983

From Pestinfo-Wiki
Revision as of 23:17, 16 February 2019 by Bernhard Zelazny (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Yabing Duan, Ying Yang, Yong Wang, Xiayan Pan, Jian Wu, Yiqiang Cai, Tao Li, Donglei Zhao, Jianxin Wang and Mingguo Zhou (2016)
Loop-mediated isothermal amplification for the rapid detection of the F200Y mutant genotype of carbendazim-resistant isolates of Sclerotinia sclerotiorum
Plant Disease 100 (5), 976-983
Abstract: The point mutation at codon 200 (TTC -> TAC, F200Y) confers moderate resistance to carbendazim in Sclerotinia sclerotiorum. This mutant genotype (F200Y) has been detected mainly by determining the minimum inhibitory concentration (MIC), which requires 3 to 5 days. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of the F200Y mutant genotype of carbendazim-resistant isolates of S. sclerotiorum. Specific LAMP primers were designed and concentrations of LAMP components were optimized. The optimal reaction conditions were 62 to 63°C for 45 min. The new LAMP assay requires no special equipment and is highly sensitive and specific (the i.e., it generated positive results with F200Y mutant genotype but generated negative results with other carbendazim-resistant mutants and with a variety of carbendazim-resistant mutants of Botrytis cinerea and Fusarium graminearum). Inclusion of the loop backward (LB) primer reduced the reaction time to 15 min. Results were identical with LAMP and MIC determinations. The advantages of the LB-accelerated LAMP assay for detection of the F200Y mutant genotype were demonstrated by assaying sclerotia produced on rape stems that were artificially inoculated in the field. The results indicated that the new LAMP assay represents an improved way to detect the F200Y mutant genotype of carbendazim-resistant isolates of S. sclerotiorum.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ming-Guo Zhou, Yabing Duan

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Botrytis cinerea
Sclerotinia sclerotiorum
Fusarium graminearum
Retrieved from "https://wiki.pestinfo.org/w/index.php?title=Plant_Disease_(2016)_100,_976-983&oldid=443769"