Journal of Plant Pathology (2017) 99, 429-435

Y. Luo, J. Zheng, T. Liu, W.M. Wang, Y. Huang, J. Shang and H. Yang (2017)
Identification and sequence analysis of a Cucumber mosaic virus isolate from Solanum lycopersicum in Southwest China
Journal of Plant Pathology 99 (2), 429-435
Abstract: Previous reports have shown the prevalence of Cucumber mosaic virus (CMV) subgroup I isolates in China. Here the complete genome of a new CMV isolate from tomato (Solanum lycopersicum) named CMV-SL was characterized and its comparative phylogenetic analysis with reference CMV isolates clustered it with subgroup II strains. CMV-SL showed high sequence identity with subgroup II isolates in RNA-1 (97-98%), RNA-2 (97-98%) and RNA-3 (95-97%). The corresponding regions were further compared. The results showed that the coding and non-coding regions of CMV-SL had high identity with other subgroup II strains. The 3'UTR of RNA-3 was the most variable region, and contained most of the observed variation. In addition, single nucleotide polymorphism (SNP) analysis of CMV is reported for the first time, showing that CMV has typical SNPs, which is a common form of genetic variation between isolates. We also estimated the omega ratios of non-synonymous and synonymous substitutions of CMV subgroup II strains. We found that negative selection was the predominant evolutionary force operating upon coding regions. However, strong positive selection was identified in the coat protein (CP) of CMV subgroup II.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus	Tomato (Solanum lycopersicum)	China (south)