Phytopathology (1998) 88, 63-69

M. Kikkert, C. Meurs, F. van de Wetering, S. Dorfmüller, D. Peters, R. Kormelink and R. Goldbach (1998)
Binding of Tomato spotted wilt virus to a 94-kDa thrips protein
Phytopathology 88 (1), 63-69
Abstract: Using protein blot assays, a 94-kDa thrips protein was identified that exhibited specific binding to tomato spotted wilt virus (TSWV) particles. Renaturation of the 94-kDa protein, which is conserved among the two major vector species of TSWV, Frankliniella occidentalis and Thrips tabaci, was crucial for its virus-binding properties, whereas under the same conditions no specific binding was observed with aphid (Myzus persicae) proteins. The 94-kDa protein species was present in all developmental stages of both vectoring thrips, whereas it was present mainly in the adult stage of a nonvectoring thrips species, Parthenothrips dracenae. Using antibodies against the different TSWV structural proteins, the G2 envelope glycoprotein was identified as the viral determinant involved. Because the virus-binding protein is present throughout the thrips body, but not in the gut, it may represent a receptor protein involved during circulation of the virus through its vector but probably not during viral uptake in the midgut.
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Database assignments for author(s): Dick Peters

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Frankliniella occidentalis
	Thrips tabaci
	Tomato spotted wilt virus