Journal of Economic Entomology (2009) 102, 2084-2089

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Noubar J. Bostanian, Serge Beudjekian, Erin McGregor and Gaétan Racette (2009)
A modified excised leaf disc method to estimate the toxicity of slow- and fast-acting reduced-risk acaricides to mites
Journal of Economic Entomology 102 (6), 2084-2089
Abstract: The 'modified excised leaf disc method' is based on leaf discs that fit tightly the bottom halves of 50-mm petri dishes. The bottom half of each petri dish is covered with wet cotton wool to prolong leaf freshness. The side wall of each bottom half has a small hole to allow the petiole of the leaf disc to protrude outside the petri dish. The top half of each petri dish has a 28-mm (diameter) window. For phytophagous mites the window is covered with a 40-µm mesh Pecap polyester screen. Using this method it was possible to estimate the LC50 value of bifenazate to be 0.00413 g (AI) /liter and the LC50 value of spirodiclofen to be 0.40050 g (AI)/liter to the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). The method reduces losses due to escapees and allows observations to be made as long as 9 d after treatment.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Noubar J. Bostanian

Research topic(s) for pests/diseases/weeds:
control - general


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Tetranychus urticae