Journal of Economic Entomology (2008) 101, 1888-1896

Xiao-Mao Zhou, Qing-Jun Wu, You-Jun Zhang, Lian-Yang Bai and Xiong-Ying Huang (2008)
Cloning and characterization of a GABA receptor from Plutella xylostella (Lepidoptera: Plutellidae)
Journal of Economic Entomology 101 (6), 1888-1896
Abstract: A full-length cDNA, with an open reading frame (ORF) of 1,449 bp, encoding a subunit of the gamma-aminobutyric acid (GABA)-activated chloride channel was isolated from Plutella xylostella (L.) (Lepidoptera: Plutellidae) (GenBank accession no. EF156251). The subunit gene encoded a 483-amino acid polypeptide that showed 84% sequence identity with DmRdl subunit (U02042) (Drosophila melanogaster resistant to dieldrin). When expressed in Xenopus laevis oocytes, the subunit assembled as a functional homomeric complex activated by GABA and abamectin in a dose-dependent manner. The EC₅₀ value of GABA was 0.49 mM (0.41-0.58) (n = 5). However, the responses to abamectin were very robust, with an EC₅₀ of 4.85 ìM (4.02-5.89) (n = 6), indicating that abamectin was >100-fold more potent in activating chloride currents than GABA. The results suggest that this subunit is vital to the formation of a functional channel and contains the binding site of abamectin.
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Link to article at publishers website
Database assignments for author(s): Qing Jun Wu

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella