Molecular Plant Pathology (2012) 13, 1149-1155

Francesca Spanu, Matias Pasquali, Barbara Scherm, Virgilio Balmas, Angela Marcello, Giuseppe Ortu, Marie Dufresne, Lucien Hoffmann, Marie-Josée Daboussi and Quirico Migheli (2012)
Transposition of the miniature inverted-repeat transposable element mimp1 in the wheat pathogen Fusarium culmorum
Molecular Plant Pathology 13 (9), 1149-1155
Abstract: High-throughput methods are needed for functional genomics analysis in Fusarium culmorum, the cause of crown and foot rot on wheat and a type B trichothecene producer. Our aim was to develop and test the efficacy of a double-component system based on the ability of the impala transposase to transactivate the miniature inverted-repeat transposable element mimp1 of Fusarium oxysporum. We report, for the first time, the application of a tagging system based on a heterologous transposon and of splinkerette-polymerase chain reaction to identify mimp1 flanking regions in the filamentous fungus F. culmorum. Similar to previous observations in Fusarium graminearum, mimp1 transposes in F. culmorum by a cut-and-paste mechanism into TA dinucleotides, which are duplicated on insertion. mimp1 was reinserted in open reading frames in 16.4% (i.e. 10 of 61) of the strains analysed, probably spanning throughout the entire genome of F. culmorum. The effectiveness of the mimp1/impala double-component system for gene tagging in F. culmorum was confirmed phenotypically for a putative aurofusarin gene. This system also allowed the identification of two genes putatively involved in oxidative stress-coping capabilities in F. culmorum, as well as a sequence specific to this fungus, thus suggesting the valuable exploratory role of this tool.
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Link to article at publishers website
Database assignments for author(s): Matias Pasquali, Quirico Migheli

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium culmorum	Wheat (Triticum)