Journal of Phytopathology (2012) 160, 509-518

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Akira Kawaguchi and Koji Inoue (2012)
New antagonistic strains of non-pathogenic Agrobacterium vitis to control grapevine crown gall
Journal of Phytopathology 160 (10), 509-518
Abstract: Graft unions of nursery stock of grapevine (Vitis vinifera L.) collected in Japan yielded non-pathogenic strains of Agrobacterium. On the basis of classic diagnostic tests, a sequence analysis and a previously reported multiplex PCR method, the non-pathogenic strains ARK-1, ARK-2 and ARK-3 were identified as Agrobacterium vitis. Stems of grapevine seedlings were inoculated with both a cell suspension of seven mixed strains of A. vitis (Ti) as a pathogen and one of a new strain or A. vitis strain VAR03-1, one of the biological control agents against crown gall previously reported, as competitors to assay the suppression of tumour formation caused by the pathogen. In a test with a 1:1 cell ratio of pathogen/nonpathogen, strains ARK-1, ARK-2 and ARK-3 reduced the tumour incidence.. In particular, strain ARK-1 was strongest at inhibiting tumour formation in this study. Strain ARK-1 established populations on roots of grapevine tree rootstock and persisted on roots for a year. ARK-1, ARK-2 and ARK-3 did not produce a halo of inhibition against A. vitis (Ti) strain on YMA medium. Moreover, strain ARK-1 did not reduce tumour incidence on the stems of grapevine when ARK-1 was dead or only culture filtrate was used. This result indicates the possibility that these new strains inhibit grapevine crown gall in planta by a different mechanism other than VAR03-1. In particular, one of the new strains, named ARK-1, was most effective in inhibiting tumour formation on grapevine and appears to be a promising new agent to control grapevine crown gall.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Akira Kawaguchi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
evaluation - screening - selection
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Rhizobium vitis Grapevine (Vitis) Japan
Rhizobium vitis (antagonist) Rhizobium vitis Grapevine (Vitis) Japan
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