Journal of Plant Pathology (1998) 80, 187-195

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G. Cirvilleri, V. Catara, G. Caldarera and P. Caruso (1998)
Genomic fingerprinting of some Pseudomonas syringae pv. pisi strains from Sicily
Journal of Plant Pathology 80 (3), 187-195
Abstract: Pseudomonas syringae pv. pisi strains were characterized by pathogenicity tests on eight differential pea genotypes and by two genomic fingerprinting methods: PCR with REP and ERIC primers, and pulsed-field gel electrophoresis (PFGE) of genomic DNA digested by rare-cutting restriction endonucleases. The study comprised reference type strains of all 7 known races and 21 strains obtained from field pea crops in Sicily. PFGE was the most discriminatory method, delineating 6 pulsed-field gel profiles not coinciding with races, whereas REP and ERIC PCR identified 2 profile types. All the P. syringae pv. pisi isolated in Sicily were identified as race 6 on the basis of pathogenicity tests, and their genomic fingerprints by PFGE and REP and ERIC PCR were identical and more closely related to the type strains of races 3 and 6 than to those of the other races. The results are discussed in relation to improvement of detection methods and race-identification protocols.
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Pseudomonas syringae pv. pisi Pea (Pisum sativum) Italy
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