REDIA (2001) 84, 7-18

G. Rotundo, G.S. Germinara and A. De Cristofaro (2001)
Sex pheromone extraction methods from individual females of Lepidoptera [Sesamia nonagrioides (Lefèbvre) (Lepidoptera Noctuidae)] by solid-phase microextraction
REDIA 84, 7-18
Abstract: Two methods to extract sex pheromone components of Lepidoptera from individual virgin in females, based on SPME technique, under heated static air conditions and from an air flow were studied. The highest recovery percentages of synthetic Z11-16:Ald, Z11-16:OH and Z11-16:Ac in heated static air were obtained by exposing an absorbent phase (polydirnethylsiloxane) for 20 min at 120°C in both 0.1 ml and 0.25 ml silanised conical vials. A flow of 40 cc/min was found optimal for the extraction of the same compounds from an air flow at 28 *C. Under more favourable conditions, the extracting methods from the air, compared to the solvent extraction and the rubbing SPME fibre method already used on Sesamia nonagrioides (Lefèbvre) (Lepidoptera Noctuidae), allowed detection of Z11-16:Ald from individual females of the Italian population, although lower quantities of Z11-16:Ac, Z11-16:OH and 16:Ac were found. All compounds were identified using GC-MS. The SPME technique, carried out in heated static air or in air flow conditions, provides two other useful methods for the identification of sex pheromone components from Lepidoptera.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Giacinto Salvatore Germinara, Antonio De Cristofaro, Giuseppe Rotundo

Research topic(s) for pests/diseases/weeds:
pheromones/attractants/traps

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sesamia nonagrioides		Italy