Pest Management Science (2020) 76, 2180-2189

Beibei Wang, Chaodu Ren, Lei Yang, Qi Fang, Qisheng Song and Gongyin Ye (2020)
Venom α-amylase of the endoparasitic wasp Pteromalus puparum influences host metabolism
Pest Management Science 76 (6), 2180-2189
Abstract:
Background
Pteromalus puparum (Hymenoptera: Pteromalidae) is an endoparasitoid wasp that parasitizes many butterfly species, including a Brassicaceae pest, Pieris rapae (Lepidoptera: Pieridae), the small white cabbage butterfly. P. puparum females inject venom along with their eggs into hosts to ensure successful parasitism. The venom regulates host development and behavior, suppresses host immunity, and influences host metabolism. It has been shown that the venom contains α-amylases, a group of hydrolytic enzymes that act in insect sugar metabolism. So far, three α-amylases have been identified in P. puparum (Pteromalus puparum α-amylases, PpAmys) and the function of PpAmy1 has been reported. However, the functions of PpAmy2 and PpAmy3 remain unknown.
Results
We studied the functions of an α-amylase highly expressed in muscle-rich tissues (PpAmy2 ) and an α-amylase highly expressed in venom apparatus (PpAmy3 ) using RNAi and GC-TOF-MS techniques. Knockdown of PpAmy3 by RNAi reduced the body length and weight of 1-day old larval offspring while there was no significant effect when PpAmy2 was knocked down. Compared to the control injected with siGFP , many metabolites in P. puparum changed when PpAmy2 was knocked down, while the injection of PpAmy3 recombinant protein into host induced metabolite changes in the P. rapae hemolymph.
Conclusion
Our study demonstrated that PpAmy2 acts in metabolism in the muscles of the parasitoid while PpAmy3 could influence the host metabolism and may support the development of parasitic wasp offspring.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Gong-Yin Ye

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pieris rapae
Pteromalus puparum (parasitoid)	Pieris rapae