Journal of Plant Pathology (2013) 95, 385-389

D. Pramesh, P. Singh and V.K. Baranwal (2013)
In vitro expression and purification of the coat protein gene of Garlic common latent virus and its application in ELISA-based diagnostics
Journal of Plant Pathology 95 (2), 385-389
Abstract: The coat protein (CP) gene of Garlic common latent virus (GarCLV) was expressed in Escherichia coli strain BL21 expression system as a 40 kDa fusion protein with histidine tag (6His) at its N and C terminals. The purified protein reacted positively in Western blot with a commercial GarCLV polyclonal antiserum and hence, used as immunogen for production of polyclonal antisera in two New Zealand white rabbits. Antisera to GarCLV (titer 1:2000) detected the virus by direct antigen-coated ELISA in GarCLV positive garlic samples. Further, the specific reactivity of the antisera was confirmed through Western blotting and immunosorbent electron microscopy. Antisera developed were successfully utilized for detection of GarCLV in 46 out of 48 different garlic accessions. The immunoreagents developed will be useful for virus indexing in garlic tissue culture programs as well as in quarantine and/or certification programs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Virendra Kumar Baranwal

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Garlic common latent virus	Onion/garlic/leek (Allium)