Journal of Plant Pathology (2009) 91, 745-750

C.J. Knight, A.M. Bailey and G.D. Foster (2009)
Agrobacterium-mediated transformation of the plant pathogenic fungus Verticillium albo-atrum
Journal of Plant Pathology 91 (3), 745-750
Abstract: We report the successful Agrobacterium tumefaciensmediated transformation of the important plant pathogen Verticillium albo-atrum. V. albo-atrum was demonstrated to be hygromycin B sensitive and consequently transformations were based on integration of either the hygromycin B resistance gene (hph) or hph in addition to the fluorescence gene DsRed. The effect of various parameters on transformation efficiency, including spore concentration, acetosyringone concentration, selection media and co-cultivation time were investigated. Transformants were analysed by PCR and Southern analysis, and were found to contain randomly integrated T-DNA that typically inserted as one or two copies. The dual marker system afforded by pCAMDsRed provides an additional and fast method of transformant verification. Development of a successful transformation system for V. albo-atrum should facilitate further molecular studies of this important plant pathogen.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Verticillium albo-atrum