Environmental Entomology (1986) 15, 1286-1291

R.E. Shade, L.L. Murdock, D.E. Foard and M.A. Pomeroy (1986)
Artificial seed system for bioassay of cowpea weevil (Coleoptera: Bruchidae) growth and development
Environmental Entomology 15 (6), 1286-1291
Abstract: An artificial seed system has been developed to evaluate putative allelochemicals and plant fractions for effects on growth and development of the bruchid, Callosobruchus maculatus (F.). The artificial seeds, which are prepared using flour from a bruchid-susceptible cowpea cultivar, permit rates of larva growth and development and survivorship comparable with those obtained with the intact parent cowpea seed. Experiments with artificial seeds containing various inhibitors of mammalian digestive proteinases demonstrated the applicability of the technique to evaluation of chemicals for effects on bruchid growth and development. Certain inhibitors (e.g., Bowman-Birk inhibitor from soybeans) have adverse effects on bruchid growth and development; others (e.g., lima bean trypsin inhibitor) have little or no effect.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Richard E. Shade, Larry L. Murdock

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Callosobruchus maculatus	Stored legumes